Seed Train Intensification Using an Ultra-High Cell Density Cell Banking Process

A current focus of biopharmaceutical research and production is seed train process intensification. This allows for intermediate cultivation steps to be avoided or even the direct inoculation a bioreactor with cells from cryovials cryobags. Based on preliminary investigations regarding suitability high cell densities cryopreservation perfusion cultivations as inoculum further cultivations, an ultra-high density working bank (UHCD-WCB) was established immunoglobulin G (IgG)-producing Chinese hamster ovary (CHO) line. The were previously expanded in wave-mixed internal filter-based 1 L volume. procedure cryovial freezing at 260 × 106 mL?1 first time. are suitable N?1 bioreactors mode. These turn can used inoculate subsequent IgG productions fed-batch mode (low-seed high-seed fed-batch) continuous comparison standard approach shows that growth antibody comparable, but time savings greater than 35% possible production.